Overview of In Vitro and In Vivo Doubled Haploid Technologies

Doubled haploids (DH) have become a powerful tool to assist in different basic research studies, and also applied research. The principal (but not the only) routine use of DH by breeding companies is produce pure lines for hybrid seed production crop species. Several decades after discovery haploid inducer maize anther culture as method plants from pollen precursors, biotechnological revolution last allowed development variety approaches pursue goal doubled production. Now, it possible DHs many species, because when does work properly, there are several others test. In this chapter, we overview currently available used using methods based on vitro culture, or involving vivo induction embryo development, combination both.